Correlation between PCNA and AgNOR scores in non-Hodgkin's lymphomas using sequential staining technique.

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Correlation between PCNA and AgNOR scores in non-Hodgkin's lymphomas using sequential staining technique.

AIMS To investigate the relation between the numbers of interphase silver stained nucleolar organiser regions (AgNORs) and immunolabelling with the monoclonal antibody PC10, which demonstrates proliferating nuclei by reacting with proliferating cell nuclear antigen (PCNA). METHODS The established sequential technique for the demonstration of interphase AgNORs and of antigens in paraffin wax s...

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DIFFERENTIATION BETWEEN MORPHOLOGICALLY AGGRESSIVE AND NON-AGGRESSIVE BASAL CELL CARCINOMA BY AgNOR STAINING

Argyrophilic staining of nucleolar organizer regions (AgNOR) has been considered to be useful in the diagnosis and prognostic evaluation of different cutaneous tumors. In order to evaluate the role of the AgNOR technique in discriminating aggressive from non-aggressive basal cell carcinoma (BCC), paraffin-embedded histologic sections from 30 cases of aggressive BCC (BCC2) and 30 cases of n...

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Surface membrane staining of immunoglobulins in paraffin sections of non-Hodgkin's lymphomas using immunogold-silver staining technique.

The immunogold-silver staining (IGSS) method is a new immunostaining technique with much enhanced sensitivity for demonstration of antigens in paraffin sections. A series of 10 non-Hodgkin's lymphomas of B cell type were stained for surface membrane immunoglobulins by the IGSS and peroxidase-antiperoxidase (PAP) methods using paraffin sections and polyclonal primary antisera. The resulting stai...

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AgNOR STAINING IN MALIGNANT AND BENIGN EFFUSIONS

Objective: To evaluate the correlation of argyrophilic nucleolar regions(AgNORs) and malignancy in benign and malignant effusions. Design: The study group consisted of pleural and peritoneal effusion samples obtained from patients suffering from various benign or malignant diseases. The cytological smears were studied by conventional haematoxylin & eosin(H&E) and silver staining for AgNORs. Set...

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DIFFERENTIATION BETWEEN BENIGN, REACTIVE AND MALIGNANT CELLS IN SEROSAL BODY FLUIDS BY AgNOR STAINING

Argyrophilic nucleolar organizer regions (AgNORs) were determined in 94 pleural, pericardial and peritoneal effusions. The results were correlated with cytologic diagnosis to determine the diagnostic potential of this technique. Cytologically the number of normal, reactive and malignant effusions were 32, 28 and 34 respectively. The mean AgNOR counts for normal, reactive and malignant spec...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1993

ISSN: 0021-9746

DOI: 10.1136/jcp.46.1.28